There are several factors that affect the success of recalling an email, such as whether the recipient has already opened it and how much time has passed since it was sent. In Outlook, the default recall time limit is typically 2 minutes, but this can vary depending on your organization's IT administrator and any custom settings they may have configured
Related Guide: How to cancel the form that has been sent for e-signature?
Once form has been cancelled, the esignature request won't be valid as well.